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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos7_AmpR

Plasmid

#119150

Purpose

Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hB

Plasmid

#60992

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
CFP-CitA6-130-Venus

Plasmid

#128016

Purpose

Unmodified FLIP CitA6-130 sensor

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Mutation

see Depositor Comments

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-B

Plasmid

#60978

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hY

Plasmid

#60995

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Cas9m3-VP64

Plasmid

#47318

Purpose

Cas9m3 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-gap16-ClopHensor

Plasmid

#26006

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

gap16-ClopHensor

Tags

Gap16 and Strep-II

Expression

Mammalian

Mutation

N-term (16AA) of Gap-43

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hR

Plasmid

#60997

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pGFH14A5

Plasmid

#39542

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 96-100 changed to alanine

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hG

Plasmid

#60994

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hC

Plasmid

#60993

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pCfB8818

Plasmid

#126909

Purpose

Template for PCR amplification of HMG1from Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

YlHMG1

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 14-3-3 epsilon K49E

Plasmid

#11945

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Tags

GST

Expression

Bacterial

Mutation

K49E

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pTH730-staRLuc

Plasmid

#38226

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHdCas9-mammalian

Plasmid

#138415

Purpose

Expression of Himar-dCas9 in mammalian cells

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMyt1(502-623)

Plasmid

#22655

Depositor

Lynn Hudson

Article

Romm et al J Neurochem. 2005 Jun . 93(6):1444-53.

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Expression

Mammalian

Mutation

contains only first 1/2 of Myt1 central domain (C…

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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