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Showing: 1301 - 1350 of 2660 results

  1. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

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  3. miniCRISPRoff-v1

    Plasmid
    #197030
    Purpose
    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized miniCRISRPoff-v1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197030-plasmid-map-sequence-id-388972

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  4. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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  5. Cas12Max

    Plasmid
    #195334
    Purpose
    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized Cas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195334-plasmid-map-sequence-id-383108

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  6. xCas12i

    Plasmid
    #195333
    Purpose
    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized xCas12i
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195333-plasmid-map-sequence-id-383109

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  7. pLenti.puro.MCV.ER.RAZ.1

    Plasmid
    #114381
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.
    Depositor
    Sudhir Krishna, Reety Arora
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MCV NCCR (viral own promoter)
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114381-plasmid-map-sequence-id-221609

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  22. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  24. ShuttleNSE

    Plasmid
    #50958
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NSE
    Available since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50958-plasmid-map-sequence-id-76714

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  37. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  38. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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  40. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  41. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  42. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid
    #104412
    Purpose
    transient expression of dCas9-dDNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    dDNMT3a
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    C706S
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104412-plasmid-map-sequence-id-204224

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  43. p-dCas9-SET7-H297G-Hygro

    Plasmid
    #104415
    Purpose
    transient expression of dCas9-dSET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    dSET7
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H297G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104415-plasmid-map-sequence-id-203185

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  44. pSNAP_24

    Plasmid
    #238199
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-FTH1(24mer) and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238199-plasmid-map-sequence-id-472676

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  45. pGFP_24

    Plasmid
    #238195
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-FTH1(24mer) and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238195-plasmid-map-sequence-id-472670

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Showing: 1301 - 1350 of 2660 results