We narrowed to 1,100 results for: PCS2

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pCS2+ GFP-2xESP3I-IRES-mCherry

Plasmid

#230996

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Type

Empty backbone

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-HRI-GFP-IRES-mCherry

Plasmid

#226099

Purpose

HRI stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Expression

Mammalian

Mutation

156-239

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 Halo-Tev- HSPB7 (zebrafish) WT

Plasmid

#108916

Purpose

Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7 (hspb7 Zebrafish)

Tags

Halo-TEV

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

Plasmid

#156411

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 EPSM V179T

Plasmid

#26998

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; V179T

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

Plasmid

#156408

Purpose

pCS2 CMV expression vector driving EYFP fused human KRAS4B

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK-E3mut

Plasmid

#29691

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK

Tags

Flag and GFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site mutated

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GSK-3beta S9A (Xenopus)/pCS2+

Plasmid

#16333

Depositor

Peter Klein

Insert

GSK-3beta (gsk3b.L Frog)

Use

Vertebrate expression

Mutation

GSK-3 with serine-9 to alanine mutation

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
GSK-3beta S9A (Human)/pCS2+

Plasmid

#16334

Depositor

Peter Klein

Insert

glycogen synthase kinase-3 beta (GSK3B Human)

Use

Vertebrate expression

Tags

Myc

Mutation

GSK-3 beta with serine-9 to alanine point mutation

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE116 pCS2+-E1A mut CBP

Plasmid

#16695

Depositor

Randall Moon

Article

Takemaru et al J Cell Biol. 2000 Apr 17. 149(2):249-54.

Insert

pCS2+-EIA mut CBP

Use

Xenopus expression

Expression

Mammalian

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
XE173 mouse Wnt-1-myc-pCS2+

Plasmid

#16851

Depositor

Randall Moon

Insert

Wnt-1 (Wnt1 Mouse)

Use

Xenopus expression

Tags

myc

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A208S

Plasmid

#27112

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; A208S

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only