We narrowed to 6,627 results for: hwa

1. pcdna3 T7Jip1(282-470)

   Plasmid

   #51703

   Purpose

   " JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted 1-281 and 471-660

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. FLAG-Mkk7-JNK2 fusion-pcw107

   Plasmid

   #64618

   Purpose

   Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Map2k7 linker MAPK9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   mouse-human fusion

   Promoter

   PGK

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

   Plasmid

   #236242

   Purpose

   Lentiviral expression of SpCas9

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   SpCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-G6-vioABE

   Plasmid

   #73438

   Purpose

   Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Talin-TS

   Plasmid

   #83376

   Purpose

   FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted between V447 an…

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMVA2RBS035

   Plasmid

   #121051

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Talin-CS

   Plasmid

   #83377

   Purpose

   Control FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted at C-terminal o…

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

   Plasmid

   #113862

   Purpose

   Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-NLuc1.1
   12GLI-Fluc
   CBF-GLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEVA228-pro4IUPi

    Plasmid

    #122018

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pro4

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 155_KRAS in pmiRGlo

    Plasmid

    #78132

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    KRAS (KRAS Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCMV-Blast mLST8-Flag

    Plasmid

    #124915

    Purpose

    For stable expression sgRNA resistant Flag tagging mLST8_

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRS-45

    Plasmid

    #40586

    Depositor

    Harald Schwalbe

    Article

    Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

    Insert

    yTAND12-TEV-hTBPc (TAF1, TBP Human, Budding Yeast)
    

    Use

    Tags

    His-tag and yTAND12

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTET-IUPi

    Plasmid

    #122019

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pTET

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgMAPKAP1_1

    Plasmid

    #124912

    Purpose

    Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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