We narrowed to 11,732 results for: Emb;

Showing: 5381 - 5400 of 11732 results

Results per page:

Sort results by:

EGFP-INPP5E-CAAX

Plasmid

#202746

Purpose

express plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

Plasmid

#180313

Purpose

bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV and sfCherry2

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MCS-eDHFR(69K6)-EGFP

Plasmid

#172101

Purpose

Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

22aa-eDHFR(69K6)-EGFP

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-shNCLX-2

Plasmid

#181871

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-shNCLX-1

Plasmid

#181870

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR322-bom

Plasmid

#155178

Purpose

pBICI_2_strong without bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP Rab4DN

Plasmid

#99019

Purpose

Localization and visualization of the dominant negative form of Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 DN (Rab4 Fly, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

S22N Dominant negative

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-SAC1-HLx10

Plasmid

#108137

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN3P-HA-Suv4-20h2mut

Plasmid

#86692

Purpose

For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)

Tags

HA

Mutation

Mutated asparagine 273 to cysteine 276 (NHDC) int…

Promoter

T3

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-MKLP1 S708/710A

Plasmid

#70153

Purpose

mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S708A, S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CHRFAM7A-YFP

Plasmid

#62634

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TMED5-bio-His

Plasmid

#53338

Purpose

Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TMED5 (TMED5 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-hERG1a S624A

Plasmid

#53056

Purpose

Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 624 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge