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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-4

Plasmid

#197814

Purpose

Level 0 J1-J4 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-11

Plasmid

#197815

Purpose

Level 0 J1-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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R777-E062 Hs.ERBB2-nostop

Plasmid

#70346

Purpose

Gateway ORF clone of human ERBB2 [NM_004448.3] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPTK013-3a-Invertase-αMFΔ

Plasmid

#84972

Purpose

Type 3a, Invertase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Invertase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK016-3-RFP_Intra

Plasmid

#84975

Purpose

Type 3, Red fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK014-3a-Killer-αMFΔ

Plasmid

#84973

Purpose

Type 3a, Killer followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Killer followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK011-3a-SA-αMFΔ

Plasmid

#84970

Purpose

Type 3a, Serum albumin followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Serum albumin followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_+nls-eGFP-HA-_+1_BK+

Plasmid

#49312

Purpose

Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-EGFP-HA

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK004-2-pTPI1

Plasmid

#84963

Purpose

Type 2, pTPI1, Triose phosphate isomerase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Triose phosphate isomerase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFunnyfarm

Plasmid

#24755

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

CAT-ccdB cassette

Use

Gateway-adapted entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-fishRab8A

Plasmid

#24904

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B

Plasmid

#24907

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ230-TREX2-N

Plasmid

#244025

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ166-TREX2-N

Plasmid

#244022

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ166-AtEXO1B-N

Plasmid

#244023

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ230-AtEXO1B-N

Plasmid

#244024

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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