We narrowed to 11,364 results for: try
-
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway CloningExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3 containing mRFP1
Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NFAT5
Plasmid#79506PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseGateway CloningExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL W117R-pBabe-puro
Plasmid#19239DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tryptophan 117 to ArgininAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L3-L2
Plasmid#62164PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseGateway CloningExpressionMammalianPromoterSV40Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-ndufs2 guide
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1-no stop
Plasmid#67345PurposehSar1 without stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mRuby3-IRES-eGtACR1-ST
Plasmid#108960PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequenceDepositorInserteGtACR1-ST
ExpressionMammalianAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_hEMSY
Plasmid#125156PurposeGateway entry cloneDepositorInsertEMSY (EMSY Human)
UseGateway CloningAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shLKB1-Hu
Plasmid#61243PurposeEntry clone encoding shRNA to human LKB1DepositorAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1-miR-30 L1-R5
Plasmid#62181PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseGateway Cloning and RNAiExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJG85
Plasmid#89281PurposeEntry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sitesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only