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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTEF6-7

Plasmid

#124583

Purpose

Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

mCherry

Expression

Yeast

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Skylan-S-(GGGGS)x3-β-Actin

Plasmid

#128938

Purpose

fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells

Depositor

Shimon Weiss

Article

Yi et al Biomed Opt Express. 2019 Apr 16;10(5):24

Insert

Skylan-S

Tags

β-actin

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2_166

Plasmid

#199098

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.0/1.1-TF

Plasmid

#234516

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-FLAG-tTA

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2_167

Plasmid

#199099

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJH1579

Plasmid

#178904

Purpose

Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

YC3.60

Expression

Worm

Mutation

See Depositor Comments Below

Promoter

Pglr-1

Available Since

Jan. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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TC1405

Plasmid

#164877

Purpose

BFP-preSTOP-GFP reporter

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

BFP-preSTOP-GFP

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFUChW nontargeting shRNA

Plasmid

#78522

Purpose

Lentiviral vector encoding shRNA and mCherry reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

mCherry

Use

Lentiviral and RNAi

Promoter

UbC

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR

Plasmid

#67930

Purpose

Translational reporter - Emi1 UTRs

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mscv2.2-6mycPrgJ

Plasmid

#60204

Purpose

6myc-tagged PrgJ (SPI-1 rod protein) from Salmonella typhimurium, under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of PrgJ

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

PrgJ

Use

Retroviral

Tags

6xMYC

Expression

Mammalian

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Fluc/CMV/WPREdU3

Plasmid

#136881

Purpose

Reporter vector expression firefly luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-9xSeq2-Luc

Plasmid

#42214

Purpose

Luciferase reporter for GI-ZFP2

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

9xSeq2 Binding Sites

Use

Luciferase

Expression

Mammalian

Promoter

9 tandem copies of Seq2 + minimal human CMV promo…

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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LENG

Plasmid

#111162

Purpose

miR-E (miR-30 variant)-based RNAi with GFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Retroviral

Tags

GFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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