We narrowed to 11,199 results for: AGA

1. pLJM1-PHD2

   Plasmid

   #223552

   Purpose

   Lentivirus transfer plasmid for expression of full length human PHD2

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE Rims2-GFP KI

   Plasmid

   #131495

   Purpose

   Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rims2, Rims2 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hRAD18 dC2-EGFP

   Plasmid

   #68826

   Purpose

   Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

   Depositor

   Satoshi Tateishi

   Article

   Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

   Insert

   human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5-PKHD1-myc

   Plasmid

   #108407

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCyCEN_Lisp2mCherry_hsp70_GFP

   Plasmid

   #137169

   Purpose

   Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

   Depositor

   Clemens Kocken

   Article

   Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

   Inserts

   mCherry
   Nanoluc
   dihydrofolate reductase
   Green Fluorescent Protein
   

   Use

   Unspecified

   Tags

   T2A

   Promoter

   P. cynomolgi hsp70 and P. cynomolgi lisp2

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pY036_ATP1A1_G5_Array

   Plasmid

   #86620

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiFC-HA-hCaspase 2 prodomain-VC155

   Plasmid

   #49261

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pBIFC-flag-hCaspase 2 prodomain-VN173

    Plasmid

    #49262

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 prodomain (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 148-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5-GFP-PKHD1

    Plasmid

    #108408

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRIG-PRAS40

    Plasmid

    #53599

    Purpose

    lentiviral expression of constitutively active PRAS40 and EGFP

    Depositor

    Heng Zhao

    Article

    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

    Insert

    PRAS40 (AKT1S1 Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and myc

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

14. STARR-seq luciferase INF enhancer vector_ORI_OAS3

    Plasmid

    #99324

    Purpose

    Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr12: 94575894 -94578286
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416GAL10-HRV-B14_3C

    Plasmid

    #203466

    Purpose

    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    HRV-B14 3C protease (HRVBgp1 )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAPM-miR30-EIF2AK2-ts1

    Plasmid

    #174247

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pbG-APRT-J-u32uni-PdTKmCh

    Plasmid

    #107279

    Purpose

    APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    APRT (APRT Human)
    puro-deltaTK; CAG::mCherry
    

    Mutation

    c.402A>T, c.407T>C

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid

    #49260

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 CARD (CASP2 Human)
    

    Tags

    Flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only