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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,693 results for: des.2

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Showing: 5421 - 5440 of 30693 results

  2. HSD17B4

    Plasmid
    #39156
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B4 (HSD17B4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. pMCSG53_N

    Plasmid
    #167255
    Purpose
    Bacterial expression of Nucleocapsid protein (residues 1-419)
    Depositor
    Alexei Savchenko
    Insert
    N protein (residues 1-419) (N SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167255-plasmid-map-sequence-id-331339

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  16. pCAG-ORF3cHA

    Plasmid
    #208653
    Purpose
    Express ORF3c-HA in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208653-plasmid-map-sequence-id-420908

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  17. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Strep
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  18. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

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  19. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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  20. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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Showing: 5421 - 5440 of 30693 results