166,367 results

1. ZsGreen1-N1

   Plasmid

   #54702

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   ZsGreen1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE7-P2A-GFP

   Plasmid

   #222996

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SV40-eGFP-Z1

   Plasmid

   #127638

   Purpose

   Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Samuel Myers, Steven Carr

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

   Viral Prep

   #105551-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   GFP

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

7. AAV.rTH.PI.Cre.SV40 (AAV Retrograde)

   Viral Prep

   #107788-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   rTH

   Tags

   None

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

   Plasmid

   #119903

   Purpose

   Bxb1 integration plasmid for cuO array with Puro selection cassette

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   cuOx144 array
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/Puro-CAG-JEDI-2P

   Plasmid

   #179458

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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