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pHR ErbB2-CD3ε-FusionRed

Plasmid

#188628

Purpose

Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ERBB2 (ERBB2 Human, Synthetic)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR2-Fv-Fvls-E

Plasmid

#15286

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR2 cytoplasmic domain FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC17A1

Plasmid

#131888

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A1 (SLC17A1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF1827

Plasmid

#143853

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARNT (ARNT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1270

Plasmid

#143942

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HLTF (HLTF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC27A3_STOP

Plasmid

#161282

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A3 (SLC27A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GNAS_pLENTI-CAG-IRES-GFP

Plasmid

#176991

Purpose

Mammalian lentiviral expression vector encoding GNAS

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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APP_pcDNA6.2/EmGFP-Bsd

Plasmid

#176954

Purpose

Mammalian expression vector encoding APP and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

APP (APP Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001145794)

Plasmid

#76210

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001147078)

Plasmid

#76211

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001147218)

Plasmid

#76212

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EIF2AK1 gRNA (BRDN0001162423)

Plasmid

#77048

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK1 (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-Chst3

Plasmid

#193024

Purpose

AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

Insert

Chst3 (Chst3 Mouse)

Use

AAV

Tags

3xFLAG and myc-tag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

Plasmid

#42650

Purpose

destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 299 SNP (TLR4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D299G

Promoter

CMV/TO

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_RNAmut

Plasmid

#73539

Purpose

Inducible lentiviral expression of Ago2_RNAmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Small RNA binding mutant PAZ domain Y311A and F31…

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Apex2 and FLag

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK2

Plasmid

#119873

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK2 (FLNB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK4

Plasmid

#119874

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK4 (FLNB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF1995

Plasmid

#144376

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KDR (KDR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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