We narrowed to 22,460 results for: KIN

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pBSFI-myr-akt-K179M

Plasmid

#49188

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

K179M

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLAMF6

Plasmid

#23396

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SLAMF6 (SLAMF6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET-6xHis-(pos15)GFP

Plasmid

#89248

Purpose

[Edit] Expresses GFP with +15 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos15)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-NBioTAP

Plasmid

#53568

Purpose

Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-CBioTAP

Plasmid

#53569

Purpose

Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEK-V5/HIS

Plasmid

#205611

Purpose

Expression of human TEK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TEK receptor tyrosine kinase (TEK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

Plasmid

#90013

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-Dicer

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PCK2

Plasmid

#20559

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCK2 (PCK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PLK2

Plasmid

#20590

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK2 (PLK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag BMX

Plasmid

#20431

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BMX (BMX Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR221_MFSD2A

Plasmid

#131893

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD2A (MFSD2A Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBluescriptSKII+ U6-sgRNA(F+E) TFRC

Plasmid

#74709

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
RAP1B

Plasmid

#55665

Purpose

expression of mouse RAP1B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP1B (Rap1b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pRV DYRK2

Plasmid

#12568

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pGBW-m4137383

Plasmid

#149541

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

H. sapiens recode;18-aa Ctrunc

Promoter

CMV

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PIK3CA - Exon20 H1047R (pFastBac)

Plasmid

#16641

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras 12V (37G)

Plasmid

#12589

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

HRas 12V 37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V, 37G

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only