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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Article

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Tags

V5

Expression

Bacterial and Mammalian

Promoter

T7 promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Article

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 Rat, with human c-terminus)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pOPTN-EGFP

Plasmid

#27052

Depositor

Beatrice Yue

Article

Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

Insert

optineurin (OPTN Human)

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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KA1775_pPBCAG-rtTAM2-IH

Plasmid

#124167

Purpose

piggyBac-based expression of rtTA-M2

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

rtTAM2-IRES-Hph

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

Plasmid

#85434

Purpose

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab

Use

Lentiviral

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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XLone-ETV2

Plasmid

#135763

Purpose

expresses human ETV2 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

ETV2 (ETV2 Human)

Expression

Mammalian

Mutation

none

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B55 alpha shRNA 3-1

Plasmid

#10679

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha shRNA (PPP2R2A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-WT

Plasmid

#30370

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

wild type

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-Y138L

Plasmid

#30378

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y138L (PTEN Human)

Expression

Mammalian

Mutation

Y138L

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-G129E

Plasmid

#30377

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-G129E (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
EF.hHES1.Ubc.GFP

Plasmid

#17624

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pFH2.10_Gag-MCP-P2A-eGFP

Plasmid

#205525

Purpose

Express HIV Gag-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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UAS:Zebrabow-B

Plasmid

#118216

Purpose

For broad gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha SNIPR

Plasmid

#188357

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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