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pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Stx4-pmCitrine

Plasmid

#92422

Purpose

Expression of syntaxin-4 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Publication

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIRECT_23A

Plasmid

#91138

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only
pLPT145

Plasmid

#85527

Purpose

Titration sponge for TetR, cI and lacI

Depositor

Johan Paulsson

Publication

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Type

Empty backbone

Promoter

PLtet, PLlac, PR

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Publication

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Publication

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

Plasmid

#85070

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet-H

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pG-dsRedxp-HIF1Beta

Plasmid

#87213

Purpose

N-terminally dsRedXP-tagged HIF-1b/ARNT

Depositor

Violaine Sée

Insert

HIF-1b (ARNT Human)

Tags

dsRedXP

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCaRNA1&2

Plasmid

#86087

Purpose

Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

Depositor

Aiming Wang

Publication

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

RNA1, RNA2

Expression

Plant

Promoter

35S

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPdR

Plasmid

#85083

Purpose

Expresses putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Publication

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin reductase

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27MC3

Plasmid

#75464

Purpose

Plasmid for constitutive expression of mcherry-pdt3

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mcherry-pdt#3

Expression

Bacterial

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP3

Plasmid

#75456

Purpose

Plasmid for constitutive expression of gfp-pdt3

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#3

Expression

Bacterial

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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PP43

Plasmid

#78953

Purpose

Figure 2. Expresses BxbI.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

BxbI

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Publication

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-erHRP(N175S mutant)

Plasmid

#79909

Purpose

erHRP(N175S mutant) pCMV backbone

Depositor

Joshua Sanes

Publication

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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