We narrowed to 59,537 results for: cloning

1. pICSL13001

   Plasmid

   #50265

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (1.3 kb) + 5'UTR, 35s (Cauliflower Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-H1-SGIPZ_NF1 sh1miR

   Plasmid

   #119206

   Purpose

   knockdown of NF1

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   sh1miR targeting NF1 (NF1 Mouse, Rat, Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE142

   Plasmid

   #120735

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   Marker_Suc2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGE250

   Plasmid

   #120746

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-5AB)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_EYK300-5AB
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCZGY2842

   Plasmid

   #87762

   Purpose

   Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Punc-25-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Punc-25

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p667-UBC-AAK1-V5-miniTurbo_IDG-K

   Plasmid

   #135255

   Purpose

   Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   AAK1-V5-miniTurbo (AAK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-miniTurbo-V5-ADCK1_IDG-K

   Plasmid

   #158195

   Purpose

   Gateway destination clone of ADCK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-ADCK1 (ADCK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL80007

   Plasmid

   #50323

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, mCherry variant of RFP (Discosoma sp.)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-CAMKK1_IDG-K

   Plasmid

   #158169

   Purpose

   Gateway destination clone of CAMKK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CAMKK1 (CAMKK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-CDK10_IDG-K

    Plasmid

    #158171

    Purpose

    Gateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK10 (CDK10 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GGE150

    Plasmid

    #120750

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_2UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P2_2UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL50007

    Plasmid

    #50308

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal FLAG tag
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GGE368

    Plasmid

    #120737

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_Nat)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_Nat
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

    Plasmid

    #119202

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Mutation

    R1276P

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

    Plasmid

    #119203

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Mutation

    R1276P

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-L3-oPRE-L2

    Plasmid

    #32415

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    oPRE
    

    Use

    Gateway cloning vector

    Mutation

    WPRE with deletion of X protein promoter and muta…

    Available Since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKOdeltagalK (Hyg)

    Plasmid

    #110090

    Purpose

    Analogous to pKO but without the negative selection gene galK.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GGE038

    Plasmid

    #120785

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Zeta-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GGE067

    Plasmid

    #120784

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Zeta-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOLUC

    Plasmid

    #12514

    Depositor

    Tong-Chuan He

    Article

    Feng et al Biotechniques. 2002 May . 32(5):992, 994

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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