We narrowed to 18,253 results for: jun
-
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only
-
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
TagsHis and MBPExpressionBacterialPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-PSD95-mCherry
Plasmid#125694PurposeExogenous expression of PSD95 (red fluorescence)DepositorInsertPSD95-mCherry
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-HRP-TM_pLX304
Plasmid#202021Purposeexpresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vectorDepositorInsertFlag-GFP-HRP-TM
UseLentiviralTagsFlag and GFPExpressionMammalianPromoterCMVAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
TagskanMXExpressionBacterialAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2_Rluc-Y0x-Fluc
Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19 tandem CAR
Plasmid#113019Purposefor lentivirus production of CD19 tandem CARDepositorInsertCD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Synthetic, Mouse)
UseLentiviralTagsGFPExpressionMammalianAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 T204D
Plasmid#80877Purposemammalian expression of constitutively active ALK5 T204DDepositorInsertALK5 (TGFBR1 Human)
TagsHAExpressionMammalianMutationT204D (constitutively active)PromoterCMVAvailable SinceAug. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
ExpressionMammalianPromoterU6+27Available SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.PAC
Plasmid#57825PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter drivenDepositorInsertssgRNA
EFS
PAC
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-FcGamma CAR
Plasmid#113014Purposefor production of lentivirus encoding the CD19 CAR-PfcgammaDepositorInsertCD19 CAR-P intracellular GFP (CD19 Human, Synthetic, Mouse)
UseLentiviralTagsGFPExpressionMammalianAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Popt
Plasmid#231705PurposeMammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV P gene (codon-optimized )
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lopt
Plasmid#231706PurposeMammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV L gene (codon-optimized )
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nopt
Plasmid#231704PurposeMammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only