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pN1-HsSmg6_1-576-V5-SBP-MBP_I

Plasmid

#146569

Purpose

Mammalian Expression of HsSmg6_1-576

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-576 (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-nCLC

Plasmid

#193564

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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nCLC-ShadowY

Plasmid

#193565

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-CLC

Plasmid

#193567

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-FKBP-EGFP

Plasmid

#193572

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC-SNAP

Plasmid

#193580

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Tags

ShadowY and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLCdeltaN

Plasmid

#193562

Purpose

Expresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Deleted amino acids 1-89

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TetO(3G)-GCaMP6f-WPRE

Plasmid

#186205

Purpose

Express GCaMP6f under TetO(3G)

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Promoter

TetO(3G)

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Topo-Tbxas1_ISH_S

Plasmid

#170307

Purpose

Encoding partial sense strand of the mouse Tbxas1 gene for in situ hybridization.

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

Partial sense strand of the mouse Tbxas1 gene for in situ hybridization

Use

For ivf

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Topo-Tbxas1_ISH_AS

Plasmid

#170308

Purpose

Encoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

Partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization

Use

For ivf

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1-RNF169UBD

Plasmid

#119009

Purpose

Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1-Rad18UBD

Plasmid

#119008

Purpose

Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMinDis.X513-iNicSnFR3b-(V7)-ER

Plasmid

#125123

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(V7)-ER

Tags

Fusion protein -Endoplasmic retention motif

Expression

Mammalian

Promoter

T7

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR3a-(CC93)

Plasmid

#124880

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(CC93)

Tags

HA Tag and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR2-(CC90)

Plasmid

#124836

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR2-(CC90)

Tags

HA tags and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-KSR R615H

Plasmid

#118332

Purpose

Expression of mouse KSR

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2017 Jan 27;292(4):1449-146

Insert

KSR R615H (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

R615H

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Tags

GAL4 activation domain

Expression

Yeast

Promoter

ADH1

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
LV-CMV-Cer-FL- Bmal L95E

Plasmid

#47374

Purpose

Bmal full length with cer tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only