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pICSL86977OD:PITG_04089

Plasmid

#173510

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_04089_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_04085

Plasmid

#173509

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_04085_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_02860

Plasmid

#173508

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_02860_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_02860a

Plasmid

#173507

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_02860_P17777

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_00582

Plasmid

#173506

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_00582_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMS22: pHelper(PmcCAST)_entry_ΔTniQ

Plasmid

#168155

Purpose

Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

Plasmid

#168152

Purpose

Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS5: pHelper(AvCAST)_entry_ΔTniQ

Plasmid

#168138

Purpose

Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS4: pHelper(AvCAST)_entry

Plasmid

#168137

Purpose

Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

Plasmid

#168134

Purpose

Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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CMV BARK R106A p2A mCherry

Plasmid

#117688

Purpose

CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs R106A peptide

Tags

HA / FLAG

Expression

Mammalian

Mutation

R106A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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DT033A

Plasmid

#159394

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlga

Plasmid

#140292

Purpose

Express soluble His-tagged zebrafish Kitlga in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MNX1wt-IDR

Plasmid

#145248

Purpose

Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MNX1wt-IDR (MNX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32ac-Flag

Plasmid

#107431

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph18

Plasmid

#133029

Purpose

Expresses Xph18 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph15

Plasmid

#133030

Purpose

Expresses Xph15 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_Neo_WT_ZFP64_CR

Plasmid

#118694

Purpose

Express sgZFP64e2 resistant cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Mutation

sgZFP64e2 targeting site was synonymous mutated

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_Neo_ZFP64_N_3XFLAG

Plasmid

#118700

Purpose

Express 3xflag tagged ZFP64

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

3XFLAG was fused to the N terminal of ZFP64 cDNA

Promoter

EF1A

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_MIER3_2

Plasmid

#86324

Purpose

Encodes gRNA for 3' target of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIER3 (MIER3 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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