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DHCR7 B6.3 gRNA

Plasmid

#90651

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B6.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DHCR7 B5.3 gRNA

Plasmid

#90650

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry-CD8-vhhGFP

Plasmid

#163930

Purpose

Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TOP2A G11.2 gRNA

Plasmid

#90914

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ANLN A2.2 gRNA

Plasmid

#90531

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R2A B5.5 gRNA

Plasmid

#90847

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2A (Guide Designation B5.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V E37G

Plasmid

#12277

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
432 pSG5L HA RB 567L

Plasmid

#10725

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB 567L (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

567L

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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GPSM2 F4.3 gRNA

Plasmid

#90699

Purpose

3rd generation lentiviral gRNA plasmid targeting human GPSM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GPSM2 (Guide Designation F4.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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GPSM2 F3.3 gRNA

Plasmid

#90698

Purpose

3rd generation lentiviral gRNA plasmid targeting human GPSM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GPSM2 (Guide Designation F3.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA2

Plasmid

#68465

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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ANAPC1 A2.3 gRNA

Plasmid

#90529

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANAPC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANAPC1 (Guide Designation A2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ANAPC1 A1.3 gRNA

Plasmid

#90528

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANAPC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANAPC1 (Guide Designation A1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CENPF C5.1 gRNA

Plasmid

#90616

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPF (Guide Designation C5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHJ425

Plasmid

#20683

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; and change…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pDsRed-HP1alpha

Plasmid

#179702

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

dsRed

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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NIPBL D8.4 gRNA

Plasmid

#90803

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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NIPBL D7.4 gRNA

Plasmid

#90802

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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