161,697 results

1. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

   Plasmid

   #228902

   Purpose

   For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

   Depositor

   Bryan Roth

   Article

   Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

   Insert

   hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
   

   Use

   AAV and Synthetic Biology

   Tags

   HA and P2A mCitrine

   Expression

   Mammalian

   Mutation

   R108K

   Promoter

   Chicken Beta Actin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA

   Plasmid

   #108746

   Purpose

   Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa271 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-myc-CUL3

   Plasmid

   #19893

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1

   Plasmid

   #72266

   Purpose

   Express gene of interest under EF-1 promoter

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. R777-E285 Hs.ROCK2

   Plasmid

   #70569

   Purpose

   Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX307-SARS-CoV-2-3CL

    Plasmid

    #160278

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

    Plasmid

    #58435

    Purpose

    Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)8-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Stephen Quake, Paul Blainey

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWT PolI

    Plasmid

    #11721

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGFP

    Plasmid

    #32748

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Insert

    GFP
    

    Use

    Tags

    DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. FosB

    Plasmid

    #187908

    Purpose

    Expresses FosB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    FosB (Fosb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV-GFP

    Plasmid

    #25999

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-GFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR_zeo_sgTP53

    Plasmid

    #183180

    Purpose

    pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE-UBC-hCD47-T2A-GFP

    Plasmid

    #205458

    Purpose

    lentiviral plasmid for expression of human CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD47 (CD47 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge