We narrowed to 113,778 results for: LAS

1. pGL4.27_GRHL-SPE

   Plasmid

   #177774

   Purpose

   Luciferase reporter for GRHL

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROSA26-promoter

   Plasmid

   #21710

   Depositor

   Philippe Soriano

   Article

   Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

   Insert

   ROSA26 promoter (Gt(ROSA)26Sor Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-PKCa-mEGFP-N1

   Plasmid

   #112269

   Purpose

   IDOCKSalpha Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   Protein Kinase C alpha (PRKCA Bovine)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   A11V (please see depositor comments below)

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBMN-2xFKBP-mEGFP-NDP52

   Plasmid

   #110465

   Purpose

   retrovirus construct for stable expressing mEGFP tagged NDP52

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Retroviral; Retrovirus

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Worm and Yeast

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pP2A_NLS_sfGFP

   Plasmid

   #63709

   Purpose

   Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
   

   Use

   Just a donor dna with nanog homologous arm for kn…

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. NLS pEGFP-N3 (1299)

   Plasmid

   #62043

   Purpose

   mammalian expression of NLS-EGFP

   Depositor

   Eric Schirmer

   Article

   Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

   Insert

   SV40 NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pNA0306

   Plasmid

   #169452

   Purpose

   pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1p

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid

    #217333

    Purpose

    piggyBac expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-alpha(740-977)-GFP

    Plasmid

    #100731

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2A1 (Ap2a1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE4-PpAPOBEC1 H122A

    Plasmid

    #138338

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1H122A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 H122A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of His122 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-EN1082_Rad21_STOP

    Plasmid

    #156450

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, RAD21 (Rad21 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEXm-GST-ATG14

    Plasmid

    #99329

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    GST-TEV tag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5myc Rac1 wt

    Plasmid

    #37030

    Depositor

    Lawrence Kirschner

    Article

    Manchanda et al Oncogene. 2013 Jul 25;32(30):3491-9. doi

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pENTR1A DHFR.cHSF1

    Plasmid

    #134737

    Purpose

    Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    DHFR

    Expression

    Mutation

    Deletion of amino acids 186-202

    Promoter

    None

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-PKCg-mEGFP-N1

    Plasmid

    #112270

    Purpose

    IDOCKSgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV115

    Plasmid

    #63182

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge