We narrowed to 17,077 results for: Por

1. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Mutation

   Promoter

   Potef

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pStr-KDEL_EPV-SBP-DsRed2-ER-5

   Plasmid

   #170676

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   EPV-SBP-DsRed2-ER-5
   streptavidin-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB194

   Plasmid

   #61154

   Purpose

   Expression of KIN28 under GAL1 promoter in yeast

   Depositor

   Charles Brenner

   Article

   Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

   Insert

   TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-hU6-mU6-Cre2GO

   Plasmid

   #136907

   Purpose

   Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Pitx2

   Plasmid

   #60668

   Purpose

   PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Pitx2 (Pitx2 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3D-Cas9n-UGI

   Plasmid

   #119137

   Purpose

   Base editor made from APOBEC3D

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3D-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBXPHM3-PCFT_NB

   Plasmid

   #165415

   Purpose

   Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

   Insert

   GgPCFT_NB (SLC46A1 Llama, Chicken)
   

   Use

   Tags

   His10-MBP-3C protease site

   Expression

   Bacterial

   Mutation

   Promoter

   arabinose

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHA-ΔN332-Kin1C

   Plasmid

   #31608

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   ΔN332 kinesin-1C (Kif5c Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPPC017.109

    Plasmid

    #171145

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrc99-Rat FABP2

    Plasmid

    #13578

    Depositor

    Martin Scanlon

    Article

    Velkov et al J Biol Chem. 2005 May 6. 280(18):17769-7

    Insert

    intestinal fatty acid binding protein (Fabp2 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pLD-hygro-EnVM

    Plasmid

    #24590

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VM

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSELt_S7.A

    Plasmid

    #125835

    Purpose

    Negative selection plasmid for evolution of an amber stop codon suppression tRNA

    Depositor

    Andrew Ellington

    Article

    Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

    Insert

    ACP-2xAmb-pheS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pr.trpL*

    Available Since

    May 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205542

    Purpose

    Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205547

    Purpose

    Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-I
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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