We narrowed to 6,140 results for: arl

1. pAAV-EF1a-sCre

   Plasmid

   #55635

   Purpose

   Expresses sCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-eGFP-Ago2

   Plasmid

   #74231

   Purpose

   GFP-Ago2 lentiviral vector

   Depositor

   Carl Novina

   Article

   Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

   Insert

   Ago2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

   Plasmid

   #130997

   Purpose

   Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   EYFP-Kv2.1

   Promoter

   Ef1a

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-hChR2(E123A)-EYFP

   Plasmid

   #35505

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-Ivy-his-hLYZ

   Plasmid

   #83920

   Purpose

   Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Ivy
   lysozyme (LYZ Human)
   

   Tags

   Ivy protein has C-terminal hisx6 tag

   Expression

   Bacterial

   Mutation

   Ivy leader sequence deleted and replaced.

   Promoter

   T7 and t7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

   Plasmid

   #202760

   Purpose

   Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   huAsCpf1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT3007

   Plasmid

   #122538

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)
   

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

   Plasmid

   #130993

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   EYFP-Kv2.1

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. yPet_cp229-Binder

    Plasmid

    #179137

    Purpose

    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    yPet_cp229

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECE HA AMPKa1 AS

    Plasmid

    #69831

    Purpose

    Expresses Expresses AMPK alpha 1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162337

    Purpose

    Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6; mU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3Basic-miR126-EGFL7-Promoter

    Plasmid

    #32244

    Depositor

    Charles Lowenstein

    Article

    Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

    Insert

    miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter region 5' UTR

    Promoter

    5' UTR

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRVdGL-1Cre

    Plasmid

    #98038

    Purpose

    Second-generation rabies viral vector genome

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    mCre
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130989

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Promoter

    CaMKIIa

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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