Addgene

We narrowed to 3,015 results for: eed

Showing: 541 - 560 of 3015 results

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pColE1_sgRNA_M1NS_AmpR

Plasmid

#119155

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT2B

Plasmid

#53110

Purpose

Expression of Drosophila 5-HT2B

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2B (CG42796 Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pEF.DEST51-mVenus

Plasmid

#154899

Purpose

Expresses a fluorescent protein, mVenus

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-3.3)/pcDNA3

Plasmid

#53595

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
CAG:: ChR2HA-2a-hM4D

Plasmid

#52520

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

Tags

2xHA on ChR2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_11/pcDNA3

Plasmid

#51987

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-4GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_150/pcDNA3

Plasmid

#51984

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-0.4)/pcDNA3

Plasmid

#53593

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Expression

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pBAC690-TEF

Plasmid

#160432

Purpose

Expresses eGFP under control of the yeast TEF2 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

S. cerevisiae TEF2 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

S. cerevisiae TEF2

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12RRFuzzy3MYB

Plasmid

#194441

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, RR, Fuzzy3MYB

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

MtU6

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-ORP1

Plasmid

#191699

Purpose

Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-PSPC1

Plasmid

#46321

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

PSPC1 (PSPC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pAG260

Plasmid

#33210

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

SYNM (SYNM Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Plasmid

#87644

Purpose

For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::SNAPf>myr::CLIPf>myr::Halo2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_300/pcDNA3

Plasmid

#51983

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-3GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9 C287A-His

Plasmid

#11830

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytic mutant (CASP9 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

C287A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-Flag-Zfp423-deltaSBD

Plasmid

#35971

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 3. ():.

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Expression

Mutation

Promoter

T7

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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