We narrowed to 55,736 results for: ran

1. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

   Plasmid

   #131507

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

   Plasmid

   #139015

   Purpose

   To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   HA-MAO

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

   Plasmid

   #185686

   Purpose

   Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-HaloTag-KKMP
   

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX408 Francisella tularensis subsp. novicida Cas9

   Plasmid

   #68705

   Purpose

   Francisella tularensis subsp. novicida Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52381

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Expression

    Mammalian

    Mutation

    deleted -3194 - -1768 upstream from OCT4 ATG

    Promoter

    OCT4

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mouse deltaPE Oct4-GFP transgenic reporter

    Plasmid

    #52382

    Purpose

    BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP pA
    Oct4 (Pou5f1 Mouse)
    PGK-gb2-Neo
    

    Expression

    Mammalian

    Mutation

    deleted -300 - -1300bp fragment upstream from Oct…

    Promoter

    Oct4 and PGK+gb2

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Expression

    Mammalian

    Promoter

    Oct4

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116955

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGL4.23-IL2RA CaRE4 SNP (plus strand)

    Plasmid

    #91851

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0346)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs61839660 C>T

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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