161,398 results

1. pPHAGE_DLD-H450A-C-TAP

   Plasmid

   #83478

   Purpose

   Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   H450A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPHAGE_DLD-S456A-C-TAP

   Plasmid

   #83479

   Purpose

   Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   S456A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

   Plasmid

   #199483

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mKate2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPS0382

   Plasmid

   #133232

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRJPaph-sYFP2

   Plasmid

   #67033

   Purpose

   Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   sYFP2
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBindCY

   Plasmid

   #158223

   Purpose

   Expressing CheZ protein in response to blue light

   Depositor

   Chueh Loo Poh

   Article

   Zhang et al J Mol Biol. 2020 May 1;432(10):3137-3148

   Insert

   CheZ
   

   Use

   Synthetic Biology

   Tags

   YbaQ

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Puro-iNgn3

   Plasmid

   #75337

   Purpose

   doxycycline inducible Ngn3 over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   neurogenin 3 (Neurog3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-SLC5A2_STOP

   Plasmid

   #161407

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A2 (SLC5A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-BFP-U6-sgRNA

    Plasmid

    #196725

    Purpose

    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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