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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSL1521 (pSPIN, pSC101* backbone)

Plasmid

#160729

Purpose

Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCnRed

Plasmid

#232908

Purpose

Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

gam (gam Synthetic, phage Lambda)

Use

Synthetic Biology

Promoter

Pbad-rha

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-LEXY (pDN122)

Plasmid

#72655

Purpose

Blue light-inducible nuclear export of mCherry

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-LEXY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Nterm-AAV

Plasmid

#194651

Purpose

N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG N-term

Use

AAV

Tags

BPNLS and NpuN spilt intein

Promoter

TNNT2

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SMC3

Plasmid

#222554

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SMC3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMC3 (SMC3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

Sophie Helaine, David Holden

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.CAP-B10

Plasmid

#175004

Purpose

non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

Depositor

Viviana Gradinaru

Article

Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

Insert

Synthetic construct isolate AAV.CAP-B10 VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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