Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,414 results for: 158

Showing: 8401 - 8430 of 10414 results

  1. pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231352
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231352-plasmid-map-sequence-id-459729

    Enlarge

  2. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231353
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231353-plasmid-map-sequence-id-459833

    Enlarge

  3. pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

    Plasmid
    #231356
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231356-plasmid-map-sequence-id-459839

    Enlarge

  4. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

    Plasmid
    #231357
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231357-plasmid-map-sequence-id-459837

    Enlarge

  5. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

    Plasmid
    #231358
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231358-plasmid-map-sequence-id-459844

    Enlarge

  6. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

    Plasmid
    #231359
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231359-plasmid-map-sequence-id-459843

    Enlarge

  13. pET-NPR4 (SA binding domain)

    Plasmid
    #226885
    Purpose
    Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form
    Depositor
    Ning Zheng
    Article
    Wang et al Nature. 2020 Oct;586(7828):311-316. doi:
    Insert
    The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    NPR4 SA-binding core (residues 373-516), with a s…
    Promoter
    T7
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226885-plasmid-map-sequence-id-449178

    Enlarge

  22. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

    Enlarge

  23. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid
    #208974
    Purpose
    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    B103-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208974-plasmid-map-sequence-id-415835

    Enlarge

  24. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid
    #208971
    Purpose
    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208971-plasmid-map-sequence-id-415826

    Enlarge

  25. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

    Enlarge

  26. mEos3.2trLATm2allL+A

    Plasmid
    #203750
    Purpose
    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATm2(allL+A) (LAT Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203750-plasmid-map-sequence-id-403335

    Enlarge

Showing: 8401 - 8430 of 10414 results