We narrowed to 32,648 results for: Eng

1. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Justin English, Fleur Ferguson

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-HA-Nrxn1α-LNS2

   Plasmid

   #128106

   Purpose

   Express HA tagged Nrxn1α-LNS2

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS2 (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-Nrxn1α-LNS4

   Plasmid

   #128108

   Purpose

   Express HA tagged Nrxn1α-LNS4

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS4 (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HA-Nrxn1α-LNS5

   Plasmid

   #128109

   Purpose

   Express HA tagged Nrxn1α-LNS5

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS5 (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pflareG-psd95-exon18

   Plasmid

   #90266

   Purpose

   Minigene reporter for alternative splicing analysis

   Depositor

   Douglas Black

   Article

   Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

   Insert

   Psd95-exon18 (Dlg4 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pflareA-psd95-exon18.

   Plasmid

   #90249

   Purpose

   Minigene reporter for alternative splicing analysis

   Depositor

   Douglas Black

   Article

   Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

   Insert

   psd95-exon18 (Dlg4 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hMLKL-Venus

    Plasmid

    #106078

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Tags

    6xR and His

    Expression

    Bacterial

    Promoter

    Pgrac and PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-flag-YTHDF2

    Plasmid

    #52300

    Purpose

    full length YTHDF2 with N-terminal flag tag for mammalian cell expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3543 pMIG Bcl-XL mut8

    Plasmid

    #8792

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G138A, R139L, I140N

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

16. GFP-Bcl-2-ΔBH4

    Plasmid

    #220300

    Purpose

    mammalian expression of Bcl-2-ΔBH4 tagged with GFP

    Depositor

    Xinlai Cheng

    Article

    Wang et al Cell Reports Physical Science, Volume 5,

    Insert

    Bcl2 (BCL2 Human)
    

    Tags

    Emerald GFP

    Expression

    Mammalian

    Mutation

    BH4 deleted

    Promoter

    CMV

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV Myc-MBP

    Plasmid

    #175103

    Purpose

    Lentiviral expression of Myc-tagged MBP

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    MBP
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO-cyclin E

    Plasmid

    #216508

    Purpose

    Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CCNE1 (CCNE1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2B-HIF1A prom

    Plasmid

    #40172

    Depositor

    Alex Minella

    Article

    Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

    Insert

    HIF1A promoter fragment (HIF1A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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