We narrowed to 32,143 results for: Eng

1. pAC92-pmax-dCas9VP96

   Plasmid

   #48224

   Purpose

   dCas9VP96 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP96 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP96

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai39

   Plasmid

   #34884

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed eNpHRv3.0-EYFP
   

   Use

   Mouse Targeting

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLNCX2-Orai1

   Plasmid

   #89818

   Purpose

   Express Orai1 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p53-(1-393)-E285K-N268D

   Plasmid

   #35089

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 with N269D and E285K mutations

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Nxph4-4Q-HA

   Plasmid

   #128104

   Purpose

   Express HA tagged Nxph4-4Q

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ai35

   Plasmid

   #34882

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed Archaerhodopsin3-GFP
   

   Use

   Mouse Targeting

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV Reep4-V5

   Plasmid

   #175120

   Purpose

   Lentiviral expression of mouse Reep4-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Reep4 (Reep4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-LyzC-GFP-U6ac-cdk2-guides

   Plasmid

   #168250

   Purpose

   "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   cdk2 sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-LyzC-RacFRET-U6ac-rac2 guides

   Plasmid

   #168248

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPC1-Intu

    Plasmid

    #65430

    Purpose

    For mammalian expression of EGFP-mouse Inturned

    Depositor

    Aimin Liu

    Article

    Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

    Insert

    Inturned (Intu Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3543 pMIG Bcl-XL mut8

    Plasmid

    #8792

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G138A, R139L, I140N

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. L-NARS-2

    Plasmid

    #166532

    Purpose

    scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L-WARS-26

    Plasmid

    #166528

    Purpose

    scFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3542 pMIG Bcl-XL mut1

    Plasmid

    #8791

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    F131V, D133A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pLV V5-nurim

    Plasmid

    #175110

    Purpose

    Lentiviral expression of V5-tagged mouse Nrm

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Nrm (Nrm Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV Tmem53-V5

    Plasmid

    #175142

    Purpose

    Lentiviral expression of mouse Tmem53-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmem53 (Tmem53 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AcrIIC1X

    Plasmid

    #128112

    Purpose

    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX (AcrIIC1 N3F/D15Q/A48I)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1-1176

    Plasmid

    #13264

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated before RSLE region

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only