We narrowed to 18,046 results for: Mut

1. pOAD eIF4G1 (1-341)

   Plasmid

   #196833

   Purpose

   Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   eIF4G1 (TIF4631 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   amino acids 1-341

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOBD2 Pab1 WT (123-204)

   Plasmid

   #196834

   Purpose

   Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOBD2 Pab1 E181R (123-204)

   Plasmid

   #196835

   Purpose

   Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids & E181R

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-NHERF1 PDZ1/2*

   Plasmid

   #195211

   Purpose

   Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

   Depositor

   David Bryant, Anthony Bretscher

   Article

   Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

   Insert

   NHERF1 (NHERF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dual PDZ1/2 GYGF>GYAA motif mutant

   Promoter

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Rtn2b-R60f-mCherry

   Plasmid

   #186607

   Purpose

   Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 mutant R60f (RTN2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   c.178insC (R60f)

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM-41P-HsXRN1_1174-1649-Strep_AH

   Plasmid

   #148901

   Purpose

   Bacterial Expression of HsXRN1_1174-1649

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

   Insert

   HsXRN1_1174-1649 (XRN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET303-ColE1-T-P110A

    Plasmid

    #180237

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domain of ColE1 …

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. KBB430

    Plasmid

    #185091

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. KBB428

    Plasmid

    #185090

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Promoter

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSN526

    Plasmid

    #184827

    Purpose

    Expresses human KIF5A(∆exon27) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Use

    Tags

    mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    polyhedrin

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYL237

    Plasmid

    #173194

    Purpose

    CRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 RNA-binding mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-Ub-VPS13D N3521S

    Plasmid

    #176463

    Purpose

    expressing VPS13D patient mutant N3521S in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    N3521S mutation

    Promoter

    CMV

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

    Plasmid

    #146124

    Purpose

    Insect Expression of DmEDC3_102-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmEDC3_102-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

    Plasmid

    #146125

    Purpose

    Insect Expression of DmEDC3_331-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmEDC3_331-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBN202

    Plasmid

    #167950

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-3xFLAG-Puro PAK5 S573N

    Plasmid

    #167195

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 S573N

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 S573N
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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