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BL21 Rosetta2 ΔrecB

Bacterial Strain

#176582

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-CAR-V5

Plasmid

#82723

Purpose

Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

coxsackievirus and adenovirus receptor (CXADR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_300/pcDNA3

Plasmid

#51983

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-3GS

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCR2.1 mNFATc2

Plasmid

#17858

Depositor

Jerry Crabtree

Article

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc2 (Nfatc2 Mouse)

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
SBFP2-anti-AlfaTag nanobody

Plasmid

#171826

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

SBFP2-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pXPR_502_sgCD45

Plasmid

#158705

Purpose

CD45 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD45 (PTPRC Human)

Use

Lentiviral

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 T23N

Plasmid

#130885

Purpose

Expresses human RAB10 T23N mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T23N

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRVdG-1mOrange2-5PSD95EGFP

Plasmid

#52482

Purpose

Expresses mOrange2 and PSD-95-EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mOrange2
PSD-95-EGFP

Use

Rabies virus

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSRP-mTAZ

Plasmid

#31795

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ

Use

RNAi and Retroviral

Promoter

RNA Pol-III

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET-A3A(N57Q)-BE3

Plasmid

#158157

Purpose

T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

Depositor

Keith Joung

Article

Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

Insert

6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS

Tags

6xHis tag on N-terminus

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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