We narrowed to 9,180 results for: mel

1. pUBC_HA_PP7-mVenus

   Plasmid

   #111268

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mVenus
   

   Use

   Synthetic Biology

   Tags

   HA, SV40 NLS, and mVenus

   Expression

   Mammalian

   Mutation

   PP7: C68S, S69G [delta]70-75 (non-aggregating)

   Promoter

   hUBC

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1_V5_mNeonGreen_FLAG_C

   Plasmid

   #218073

   Purpose

   RESOLUTE cloning plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-mNeonGreen-V5

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199195

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21c(+)-mtPheRS

   Plasmid

   #31080

   Depositor

   Linda Spremulli

   Article

   Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

   Insert

   Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   T7

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pRGEB-Cas9-VirD2 (PCO)

   Plasmid

   #139802

   Purpose

   Expressing Cas9-VirD fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   Cas9-VirD2
   

   Tags

   Cas9-VirD2

   Expression

   Plant

   Promoter

   Ubiquitin

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVimentin-miRFP703

   Plasmid

   #79996

   Purpose

   Vimentin labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   Vimentin (VIM Human)
   

   Tags

   miRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT TO myc hSeparase

   Plasmid

   #59820

   Purpose

   Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ERBB4 (kinase dead)

   Plasmid

   #29533

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Expression

   Mammalian

   Mutation

   changed lysine 751 to methionine

   Available Since

   July 20, 2011

   Availability

   Academic Institutions and Nonprofits only

9. MMP11-bio-His

   Plasmid

   #53408

   Purpose

   Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   MMP11 (MMP11 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. All-in-one split-ADAR2(E488Q, N496F)

    Plasmid

    #170148

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD_His B_QuasAr1

    Plasmid

    #64135

    Purpose

    Fluorescent reporter for membrane voltage with improved brightness

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr1
    

    Expression

    Bacterial

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Luciferase-pcw107

    Plasmid

    #64648

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. TOPO tTR-KRAB-rtTA3-Luc

    Plasmid

    #68449

    Purpose

    TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    

    Use

    Luciferase; Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabePuro-DNAJA4CDS

    Plasmid

    #43799

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    DNAJA4 CDS (DNAJA4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCDF1-ERBB4 (kinase dead)

    Plasmid

    #29544

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

17. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

    Plasmid

    #75132

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pRGEB-VirD2-Cas9

    Plasmid

    #139803

    Purpose

    Expressing VirD-Cas9 fusion under ubiquitin promoter

    Depositor

    Magdy Mahfouz

    Article

    Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

    Insert

    VirD2-Cas9
    

    Tags

    VirD2-Cas9

    Expression

    Plant

    Promoter

    Ubiquitin

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 hML23Cm6

    Plasmid

    #109368

    Purpose

    Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)
    

    Tags

    1D4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDsred monomer C1- MLPH

    Plasmid

    #89236

    Purpose

    Expression of Dsred-tagged human Melanophilin in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    Human Melanophilin (MLPH Human)
    

    Tags

    Dsred monomer

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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