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Showing: 5721 - 5740 of 67199 results

  2. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  3. CoV2-Spike-D614G-Epsilon

    Plasmid
    #177964
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)
    Depositor
    Melanie Ott
    Publication
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
    Expression
    Mammalian
    Mutation
    S13I, W152C, L452R, D614G
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177964-plasmid-map-sequence-id-348252

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  4. PHPT1A

    Plasmid
    #42360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHPT1A (PHPT1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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  8. Zhou-COVID-19-Spike

    Plasmid
    #161029
    Purpose
    Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.
    Depositor
    Dapeng Zhou
    Publication
    Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:
    Insert
    Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
    Expression
    Mammalian
    Mutation
    Codon optimized
    Available since
    Jan. 12, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161029-plasmid-map-sequence-id-320615

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  18. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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Showing: 5721 - 5740 of 67199 results