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pJZC620

Plasmid

#62282

Purpose

Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
PCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been inactivated by mutatio…

Promoter

pAdh and pTdh3

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9

Plasmid

#60551

Purpose

Expresses CibN-dCas9 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

CibN-dCas9

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP)

Plasmid

#14043

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pSV human p55 alpha (HA tag)

Plasmid

#11498

Depositor

Ronald Kahn

Article

Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

Insert

p55 alpha (PIK3R1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pLX304_zeo_mmIrf1

Plasmid

#160098

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pLenti X2 Blast/shp16 (w112-1)

Plasmid

#22261

Depositor

Eric Campeau

Insert

sh cyclin-dependent kinase inhibitor 2A

Use

Lentiviral and RNAi

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-del C

Plasmid

#33095

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted C terminal (amino acids 291-450)

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KB1

Plasmid

#23686

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-RCKW

Plasmid

#25064

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase-WD40 domains …

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Article

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s(alpha3beta5/G226A/A366S)

Plasmid

#55797

Purpose

This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

Depositor

Catherine Berlot

Article

Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

Insert

alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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K9L mutant of H3K9me3 biosensor

Plasmid

#120807

Purpose

FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)

Expression

Mammalian

Mutation

lysine 9 is mutated to leucine 9 on histone H3

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMM287

Plasmid

#46035

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN K577M (WRN Human)

Use

Teton, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

K577M helicase-dead mutant; S2G introduced during…

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Tags

VSV and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (2O-T)

Plasmid

#29710

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Mocr-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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p405GAL1

Plasmid

#15963

Depositor

Nicolas Buchler, Fred Cross

Insert

p405GAL1 (GAL1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GAL1 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Article

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Tags

GFP and His

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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