We narrowed to 17,290 results for: ADA

1. pcDNA-ET-L630W

   Plasmid

   #72543

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L630W

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBItetDT960GFP

   Plasmid

   #80419

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. W118-1_hTGM2-Flag

   Plasmid

   #180407

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-OsTIR1(F74G)

   Plasmid

   #140730

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynapsin1.OxLight1

   Plasmid

   #169792

   Purpose

   Fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight1
   

   Use

   AAV

   Promoter

   human Synapsin-1

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NC16 pCDNA3.1 FLAG NRF2

   Plasmid

   #36971

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   NRF2 (NFE2L2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAJ075_hsCRBN

   Plasmid

   #124214

   Purpose

   Insect cell expression vector for His6 tagged hsCRBN

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   Cereblon (CRBN Human)
   

   Tags

   His6-TEV

   Expression

   Insect

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX6P1 p37

    Plasmid

    #169015

    Purpose

    Expresses GST-p37 (human, codon optimized for expression in bacteria)

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    p37 (UBXN2B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    sequence was codon optimized for expression in E.…

    Promoter

    tac

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2R3a-TagRFP-OcsT

    Plasmid

    #71270

    Purpose

    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330A-nBRD4/PITCh

    Plasmid

    #91794

    Purpose

    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    SpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159816

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Ryota Uehara, Gohta Goshima

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-DCX-TurboID-V5

    Plasmid

    #190740

    Purpose

    Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    doublecortin (DCX Human)
    

    Tags

    TurboID and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBItetDMPKSGFP

    Plasmid

    #96903

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Expression

    Mammalian

    Promoter

    tetracycline responsive and bidirectional

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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