Addgene

We narrowed to 1,961 results for: IPP

Showing: 841 - 870 of 1961 results

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pACYCDuet-1:ChrA-C66S

Plasmid

#225081

Purpose

Expresses precursor peptide ChrA-C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C66S

Use

Tags

His6

Expression

Bacterial

Mutation

C66S

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-NUDT5

Plasmid

#219955

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

NUDT5 (NUDT5 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-LAP3

Plasmid

#217661

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-LAP3

Plasmid

#217660

Purpose

For gateway cloning of LAP3

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Use

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH-ChrI

Plasmid

#225083

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD1

Plasmid

#172572

Purpose

For gateway cloning of N-terminal tagged MACROD1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-YTH-EGFP

Plasmid

#209322

Purpose

AAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTH-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1-YTH

Plasmid

#209319

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTH-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10D(E215Q)-HA

Plasmid

#209248

Purpose

Mammalian expression of ATP10D mutant E215Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E215Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10D(E215Q)-HA

Plasmid

#209260

Purpose

Mammalian expression of ATP10D mutant E215Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E215Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B4-HA

Plasmid

#209229

Purpose

Mammalian expression of ATP8B4

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10B(E210Q)-HA

Plasmid

#209247

Purpose

Mammalian expression of ATP10B mutant E210Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10B (ATP10B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E210Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10B-HA

Plasmid

#209233

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10B (ATP10B Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10B(E210Q)-HA

Plasmid

#209259

Purpose

Mammalian expression of ATP10B mutant E210Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10B (ATP10B Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E210Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10A-HA

Plasmid

#209232

Purpose

Mammalian expression of ATP10A

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B3-HA

Plasmid

#209228

Purpose

Mammalian expression of ATP8B3

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B3 (ATP8B3 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD1

Plasmid

#172578

Purpose

For generation of FlpIn lines with untagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

Plasmid

#172583

Purpose

For transient expression of C-terminal BirA-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD2

Plasmid

#172573

Purpose

For gateway cloning of N-terminal tagged MACROD2 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Use

Tags

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 314

Plasmid

#98584

Purpose

pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Inserts

CsrA
csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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tdEos-Cx43-7

Plasmid

#57603

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Cx43

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET14b-PITPalpha N90F

Plasmid

#58257

Purpose

bacterial expression of human PITP alpha N90F

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

N90F (abolishes PI binding)

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only