We narrowed to 2,891 results for: gem

1. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

   Plasmid

   #225441

   Purpose

   Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

   Depositor

   Diego Orzaez

   Insert

   hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEM-human TCR zeta/2470

   Plasmid

   #11507

   Depositor

   Allan Weissman

   Article

   Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

   Insert

   TCR zeta (CD247 Human)
   

   Use

   General cloning

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

3. 3155 pGEM-4Z Bcl-2 cDNA G145A

   Plasmid

   #8751

   Depositor

   Stanley Korsmeyer

   Article

   Yin et al Nature 1994 May 26;369(6478):321-3.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glycine 145 mutated to Alanine

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pGEM-rat FceRI beta cDNA

   Plasmid

   #8374

   Depositor

   Jean Kinet

   Article

   Kinet et al Proc Natl Acad Sci U S A 1988 Sep;85(17)

   Insert

   rat Fc epsilon RI beta chain (Ms4a2 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pGEM-mouse TCR zeta/2451

   Plasmid

   #11506

   Depositor

   Allan Weissman

   Article

   Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

   Insert

   TCR zeta (Cd247 Mouse)
   

   Use

   General cloning

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

6. XE169 mouse Wnt-1-myc-pGEM4

   Plasmid

   #16855

   Depositor

   Randall Moon

   Article

   McMahon et al Cell. 1989 Sep 22;58(6):1075-84. doi: 10

   Insert

   Wnt-1-myc (Wnt1 Mouse)
   

   Use

   Xenopus expression

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

   Plasmid

   #205762

   Purpose

   Single-color FUCCI4 senor for transitions of all four cell cylce phases

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

   Plasmid

   #193139

   Purpose

   Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119067

   Purpose

   Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119065

    Purpose

    Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197061

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197065

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    I3-01
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197064

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197062

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197063

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid

    #92139

    Purpose

    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRE-pfv-Sapphire

    Plasmid

    #231957

    Purpose

    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

    Depositor

    Michael Shtutman

    Article

    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

    Insert

    genetically encoded multimeric nanoparticles
    

    Use

    Lentiviral; Doxocycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAN1232

    Plasmid

    #127164

    Purpose

    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)

    Depositor

    Hana El-Samad

    Article

    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUBC-Pfv-Sapphire

    Plasmid

    #203649

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TetCMV-Pfv-Sapphire

    Plasmid

    #203650

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge