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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Publication

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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IL6-GFP

Plasmid

#28088

Depositor

Jennifer Stow

Publication

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

IL6 (Il6 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Publication

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Publication

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

Lifeact-Dendra2

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCAGMKOSiE

Plasmid

#20865

Depositor

Keisuke Kaji

Publication

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)

Expression

Mammalian

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Publication

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Publication

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pmCherry-CIBN-CreC

Plasmid

#26889

Purpose

Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

Depositor

Chandra Tucker

Publication

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN-CreC

Expression

Mammalian

Mutation

aa 1-170 of CIB1, aa 106-343 of Cre

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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GFP-RB FL

Plasmid

#16004

Depositor

Olimpia Meucci

Publication

Khan et al Cell Death Differ. 2008 Oct . 15(10):166

Insert

Rb (RB1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-SF2

Plasmid

#17990

Depositor

Tom Misteli

Publication

Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

Insert

SF2 (SRSF1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains SF2 (aa1-210)

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EGFP-E-Syt1

Plasmid

#66830

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pENTR4-SNAPf (w878-1)

Plasmid

#29652

Depositor

Eric Campeau

Type

Empty backbone

Use

Gateway Cloning

Tags

SNAPf

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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