We narrowed to 41,592 results for: Eng

1. GST-FAST-FH

   Plasmid

   #16524

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   FAST-1 (FH domain) (FOXH1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-150. Forkhead domain.

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA-H1-sgRNA-hTZAP

   Plasmid

   #87186

   Purpose

   guideRNA targeting exon1 of human TZAP

   Depositor

   Eros Lazzerini Denchi

   Article

   Li et al Science. 2017 Jan 12. pii: aah6752. doi:

   Insert

   TZAP (ZBTB48 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PI(DM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87589

   Purpose

   PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Use

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b+PCNA2 L171C

   Plasmid

   #66177

   Purpose

   Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   L171C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b+PCNA1 G108C

   Plasmid

   #66140

   Purpose

   Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C

   Promoter

   T7 promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL3335

   Plasmid

   #42798

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-park2-right (prkn Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

7. TAL3334

   Plasmid

   #42797

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-park2-left (prkn Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pMOD_A1310

   Plasmid

   #91034

   Purpose

   Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9_H840A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   H840A

   Promoter

   ZmUbi

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VV220: CoChR(C108S)-eGFP in fck

   Plasmid

   #58512

   Purpose

   Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   CoChR(C108S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 108 to Serine

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW213J116-Amp32C

    Plasmid

    #78648

    Purpose

    pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J116-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMOD_A0208

    Plasmid

    #91004

    Purpose

    Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EC1.2

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPC-Flag-TZAPZNF9-11

    Plasmid

    #87184

    Purpose

    retroviral stable expression of truncation protein Flag-TZAPZNF9-11

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ptp4a3_R (TAL 3001)

    Plasmid

    #35993

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting ptp4a3 (ptp4a3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. miR-144-451 Trap

    Plasmid

    #61488

    Purpose

    to knockdown endogenous miR144 and miR451 levels

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-144-451 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pENTR221-WNT3A

    Plasmid

    #79501

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT3A (WNT3A Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-SMVP-Cas9N-U6-gRNA M4

    Plasmid

    #80937

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCLXSN-ING2

    Plasmid

    #13293

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. mPapaya1-Nup50-N-10

    Plasmid

    #56355

    Purpose

    Localization: Nuclear Pore, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Nup50 (NUP50 Human)
    

    Use

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-Flag-YAP-S387A

    Plasmid

    #33071

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S387A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pSB77

    Plasmid

    #19590

    Depositor

    Jay Keasling

    Article

    Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

    Insert

    thiosulfate reductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only