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Lenti-LbCpf1-Blast

Plasmid

#84751

Purpose

Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

LbCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Promoter

EFS-NS

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159845

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAC8_MF (VE5596)

Plasmid

#139768

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Type

Empty backbone

Promoter

PH or p10

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pIL6-Nluc-hbb

Plasmid

#239843

Purpose

Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGG-NFIX2

Plasmid

#112699

Purpose

Expression of NFIX2 protein and GFP

Depositor

Richard Gronostajski, Francois Guillemot

Article

Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

Insert

Nuclear Factor I X2 (Nfix Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

Plasmid

#160611

Purpose

Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:LbCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Clathrin-15

Plasmid

#58005

Purpose

Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-TEV-TBP6.9F34MF37MF77M

Plasmid

#168268

Purpose

Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

TBP6.9

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F34M, F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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psigma1-EGFP

Plasmid

#53611

Purpose

Expresses sigma1 of adaptor protein of AP1

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma 1 (Ap1s1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMX GFP Rac G12V

Plasmid

#14567

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

Rac1 G12V (RAC1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

G12V

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-VdC9BV

Plasmid

#62195

Purpose

Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

Depositor

Kam Leong

Article

Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

Insert

VP64dCas9BFPVP64

Use

Lentiviral

Tags

Two VP64s tagged to dCas9 fused to BFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive) Cas9 (dCas9)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS puro human-GFP-RLIM-IRES-mCherry

Plasmid

#232243

Purpose

Mammalian expression of RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

RLIM-GFP-IRES-mCherry (RLIM Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP C1

Plasmid

#202424

Purpose

Encodes GFP alone

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-146

Plasmid

#118562

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-ER-5

Plasmid

#55418

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

ER (CALR Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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