We narrowed to 166,131 results for: addgene

1. pMB204: UbC-GFP-Tg_SAP30L-WPRE

   Plasmid

   #168109

   Purpose

   Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   SAP30L (SAP30L T. guttata)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. THI ONLY pALD4 T-

   Plasmid

   #161497

   Purpose

   Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2 (non activation control NAC)
   gRNA (Non targeting control NTC)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK434

   Plasmid

   #155378

   Purpose

   PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A4NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK422

   Plasmid

   #155373

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK423

   Plasmid

   #155374

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Oct4deltaPE-53BP1mCherry

   Plasmid

   #141122

   Purpose

   Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

   Depositor

   John Schimenti

   Article

   Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

   Insert

   Fragment of human 53BP1 (TP53BP1 Human)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJS249

   Plasmid

   #134146

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   pHis-Precission-human Ska1 MTBD (SKA1 Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDK616

   Plasmid

   #134202

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Expression

   Bacterial

   Mutation

   K223A K226A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZS160

    Plasmid

    #134238

    Purpose

    Lentiviral plasmid expressing sgRNA against HJURP

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Human HJURP sgRNA
    

    Use

    Lentiviral

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKM328

    Plasmid

    #134243

    Purpose

    Mammalian expression construct expressing sgRNA against PLK1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    gRNA targeting PLK1 3'
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIW087

    Plasmid

    #134128

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K183A K184A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIW090

    Plasmid

    #134129

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K223A K226A

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miR-722 pCDNA

    Plasmid

    #97162

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-722 (mir722 Zebrafish)
    

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FH95pUP95S73AGW (F2)

    Plasmid

    #74039

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

    Depositor

    Robert Malenka, Bernardo Sabatini, Weifeng Xu

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    H1

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gh161

    Plasmid

    #106825

    Purpose

    expression of gRNA targeting TMTC2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    TMTC2 (TMTC2 Human)
    

    Expression

    Mammalian

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLK4 G8.1 gRNA

    Plasmid

    #90837

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK4 (Guide Designation G8.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge