We narrowed to 20,522 results for: LEA
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Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
ExpressionBacterialMutationA52VAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl-MBP
Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
ExpressionBacterialAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCLX-UBI-Tred
Plasmid#27246DepositorInsertTurboRFP
UseLentiviralAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA
Plasmid#129708PurposeExpress cre-dependent KASH-EGFP and scramble shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9-D10A
Plasmid#61477Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variantDepositorInsertCas9-D10A
UseCRISPRExpressionPlantMutationcatalytic Asp10 changed to AlaPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemExpressionMammalianMutationY64L (mutRFP)Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-1m
Plasmid#13540DepositorInsertFLIP-E A207W
TagsECFP and VenusExpressionBacterialMutationA207W in binding proteinAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI
Plasmid#226009PurposeDjCas13d guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU398
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PEP102E
Plasmid#97388Purposebinary construct of nuclear-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
TagsNuclear targetExpressionPlantAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pαH-S-RRAR
Plasmid#164569PurposeSARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)DepositorInsertSpike (S-RRAR variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)
Plasmid#85179PurposeFor nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear localization signal (nls) tagRFP
ExpressionMammalianAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Reporter
Plasmid#36008DepositorInsertLAP2β-TM
UseReporterTags11kD FRB and EGFPExpressionMammalianMutationFRB is residues 2025-2114 of FRAP, LAP2β-TM is re…PromoterCMVAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
nLuc_AP4_TEVs_P3mS
Plasmid#119299PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logicDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsplit nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
UseLuciferaseTagsMyc tagExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NLS
Plasmid#118937PurposeUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
ExpressionPlantAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only