We narrowed to 16,419 results for: LEA

Showing: 5821 - 5840 of 16419 results

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pAAV-hSyn-STChRger2-TS-EYFP

Plasmid

#129395

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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VAMP4-SEP

Plasmid

#174406

Purpose

Reporter for VAMP4 exocytosis in mammalian cells

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (VAMP4 Human)

Tags

superecliptic phulorin (mutated GFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AV13_pCAG.Cas9.gRNA.NT

Plasmid

#199260

Purpose

Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
Non-targeting (NT) control gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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EF12J

Plasmid

#42943

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN+ Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pExpreS2-1-GFP

Plasmid

#175363

Purpose

ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Insert

sgGFP

Expression

Insect

Mutation

F64L, S65C, and I167T

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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PET-WT SpCas9-NLS-6xHis

Plasmid

#207373

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

WT SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-JNK1

Plasmid

#86830

Purpose

mammalian expression of GFP-JNK1

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK1 (MAPK8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Ahi1

Plasmid

#30494

Depositor

Joseph Gleeson

Article

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0mut

Plasmid

#208718

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT2h

Plasmid

#208709

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Promoter

CamKIIa

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Nucbow

Plasmid

#158992

Purpose

Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Nucbow

Tags

All FPs are fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSOMA-NLS

Plasmid

#118937

Purpose

Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal

Expression

Plant

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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myristoylated ct-GRK2

Plasmid

#178734

Purpose

N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

Depositor

Ege Kavalali

Article

Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

Insert

GRK2 (GRK2 Bovine)

Use

Lentiviral

Tags

Src 1-15

Mutation

only amino acids 548-671

Promoter

Ubiquitin

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HTmut

Plasmid

#208720

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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