We narrowed to 6,957 results for: mag

1. G protein alpha-s-mCherry

   Plasmid

   #66968

   Purpose

   G protein alpha-s internally tagged with mCherry and EE epitope

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   G-alpha-s-EE-mCherry (Gnas Rat, Discosoma sp.)
   

   Use

   Tags

   internal EE epitope (residues 189-194 in alpha- s…

   Expression

   Mammalian

   Mutation

   BamHI sites in the alpha-s cDNA were removed and …

   Promoter

   CMV

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Desmoplakin I donor only control (F40-based tension sensor)

   Plasmid

   #119188

   Purpose

   The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

   Plasmid

   #192785

   Purpose

   Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and mXFPe

   Expression

   Mammalian

   Mutation

   mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

   Plasmid

   #74306

   Purpose

   Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Inserts

   TRPV4 (Trpv4 Rat)
   ferritin (FTL and HTL1) (FTL, FTH1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG and p2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (after cre recombination)

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-mEGFP-RAX-MUT

   Plasmid

   #194573

   Purpose

   Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   RAX (RAX Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   S222Rfs*63

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-mEGFP-MYOD1-MUT

   Plasmid

   #194571

   Purpose

   Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   R188Pfs*90

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-AlbEx14-mNG

   Plasmid

   #201780

   Purpose

   knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

   Depositor

   Hajime Hirase

   Article

   Vittani et al Fluorescence Imaging of the Brain. Neuro

   Inserts

   Alb gRNA (Alb Mouse)
   Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-rMERTK-HITI

   Plasmid

   #87119

   Purpose

   Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

   Plasmid

   #158758

   Purpose

   Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   nullCoChR-GCaMP6f-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G protein alpha-s-mCerulean

    Plasmid

    #66084

    Purpose

    G protein alpha-s internally tagged with mCerulean and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid

    #118717

    Purpose

    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 18065-M02-412

    Plasmid

    #225665

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsmCherry
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AURKA-Aquamarine

    Plasmid

    #228564

    Purpose

    Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Use

    Tags

    Aquamarine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-SAP102 [N19/2R-2b]

    Plasmid

    #219474

    Purpose

    Mammalian Expression Plasmid of anti-SAP102 MAGUK scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N19/2.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-SAP102 MAGUK scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Dlg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-PSD-95 [K28/77R]

    Plasmid

    #220380

    Purpose

    Mammalian Expression Plasmid of anti-PSD-95 MAGUK scaffolding protein (Human) IgG2a R-mAb. Derived from hybridoma K28/77.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-PSD-95 MAGUK scaffolding protein (Homo sapiens) recombinant mouse monoclonal antibody. (DLG4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid

    #196887

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer 21-3xFlag-hMEFV-ΔPLD

    Plasmid

    #195479

    Purpose

    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain) with a 3xFlag tag at the N-terminus

    Depositor

    Thomas Henry

    Article

    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    deletion of residues 88 - 310 (the resulting pyri…

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid

    #192704

    Purpose

    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…

    Expression

    Mammalian

    Mutation

    meGFPm: gluatmic acid 142 to asparagine and histi…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid

    #192784

    Purpose

    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TRE-FRT-2xtTA

    Plasmid

    #191205

    Purpose

    Flpo dependent tTA expression under the control of TRE promotor

    Depositor

    Janelia Viral Tools

    Article

    Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

    Insert

    2xtTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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