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pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAK02

Plasmid

#19001

Depositor

Jay Keasling

Article

Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

Insert

arabinose transporter araE under control of Ptac (araE E. coli)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-APIC-LexAGAD

Plasmid

#112807

Purpose

gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_TALEN-FokI(WT)-BB_V3

Plasmid

#42588

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

TALEN-FokI(WT)-BB

Use

AAV; Adeno-associated virus ()

Tags

3XFlag

Expression

Mammalian

Promoter

CAG hybrid

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pReporter_9

Plasmid

#60569

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_9

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pTrc_AraC-I113-LOV

Plasmid

#206804

Purpose

Expresses AraC-I113-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

Insert

AraC-AsLOV2 fusion

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc_AraC-S170-LOV

Plasmid

#206805

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

Insert

AraC-AsLOV2 fusion

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2B -376/+64

Plasmid

#24945

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_LUC

Plasmid

#136083

Purpose

Firefly luciferase

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_LUC

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2Y641,645,646F

Plasmid

#22638

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 Y641,645,646F (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 641, 645 and 646 to Phenylalanine

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pMEXC3GH (Apr)

Plasmid

#110083

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-Bba_K822000

Plasmid

#74094

Purpose

L-Lactate inducible promoter from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_K822000 (L-Lactate promoter - E. coli MG1655)

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2_174

Plasmid

#199106

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_177

Plasmid

#199109

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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