We narrowed to 18,974 results for: IRE

1. pAPM-miR30-DDX17-ts3

   Plasmid

   #174240

   Purpose

   DDX17 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   DDX17 shRNA (DDX17 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TLNRD1(4H)-2T_pET47b

   Plasmid

   #224069

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   L191T/A225T double mutant; reduces affinity of TL…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. TLNRD1(4H)-2E_pET151

   Plasmid

   #224068

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS; V5

   Expression

   Bacterial

   Mutation

   K192E/R233E double mutant; prevents actin binding…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

   Plasmid

   #206434

   Purpose

   Vasa 463-661 expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Vasa 463-661 (vas Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   F504E

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

   Plasmid

   #206464

   Purpose

   Oskar 139-240 mut expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Oskar 139-240 mut (osk Fly)
   

   Use

   Tags

   PH mcherry

   Expression

   Insect

   Mutation

   A162E L228E)

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB-cT3G-cERP2-SOX17

   Plasmid

   #222925

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

   Plasmid

   #220494

   Purpose

   To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting LIG4 exon 3 (LIG4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-TEX264wt-cFlag

   Plasmid

   #220204

   Purpose

   Mammalian expression of wild type TEX264, C-terminal Flag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-TEX264-E194A-cFlag

   Plasmid

   #220207

   Purpose

   Mammalian expression of TEX264 with E194A mutation, C-terminal Flag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   194E>A

   Promoter

   CMV

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti-KRAS-G121C/G13A-nick sgRNA

    Plasmid

    #214099

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    KRAS G12C or G13A nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1-iPE2-Hygro

    Plasmid

    #214019

    Purpose

    inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAN056

    Plasmid

    #220052

    Purpose

    Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR (exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAN057

    Plasmid

    #220053

    Purpose

    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.3846G>A mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2252

    Plasmid

    #143581

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF630 (ZNF630 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF0009

    Plasmid

    #143197

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF708 (ZNF708 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF2500

    Plasmid

    #142117

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PML (PML Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF0371

    Plasmid

    #142609

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFYC (NFYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Amber-free HIV-1 deltaRT

    Plasmid

    #213007

    Purpose

    An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

    Depositor

    Maolin Lu

    Article

    Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

    Inserts

    HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
    Env BG505
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSII-PPM1H-mApple-mito

    Plasmid

    #207351

    Purpose

    Mammalian expression of PPm1H-mito

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPm1H-mito (PPM1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CCM2_I432D_eGFP

    Plasmid

    #208879

    Purpose

    Express eGFP-CCM2 with the I432D mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I432D

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CCM2_W421AD422A_eGFP

    Plasmid

    #208880

    Purpose

    Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations W421A and D422A

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CCM2_I428S_eGFP

    Plasmid

    #208878

    Purpose

    Express eGFP-CCM2 with the I428S mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I428S

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV5D HA-PPM1H S124A

    Plasmid

    #208368

    Purpose

    Mammalian expression of HA-tagged human PPM1H S124A

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    HA-PPM1H S124A (PPM1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S124A

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV5D HA-PPM1H S211A

    Plasmid

    #208369

    Purpose

    Mammalian expression of HA-tagged human PPM1H S211A

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    HA-PPM1H S211A (PPM1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S211A

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-NIP45 wt

    Plasmid

    #210266

    Purpose

    Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-NIP45 wt

    Plasmid

    #210262

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF1003

    Plasmid

    #143089

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF300 (ZNF300 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF0231

    Plasmid

    #143207

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF595 (ZNF595 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

    Plasmid

    #174895

    Purpose

    amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV5D HA-Δ10 PPM1H

    Plasmid

    #207335

    Purpose

    Mammalian expression of HA-Δ10 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ10

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV5D HA-Δ20 PPM1H

    Plasmid

    #207336

    Purpose

    Mammalian expression of HA-Δ20 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ20

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5D HA-Δ34 PPM1H

    Plasmid

    #207337

    Purpose

    Mammalian expression of HA-Δ34 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ34

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV5D HA-Δ37 PPM1H

    Plasmid

    #207338

    Purpose

    Mammalian expression of HA-Δ37 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ37

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV5D HA-Δ44 PPM1H

    Plasmid

    #207339

    Purpose

    Mammalian expression of HA-Δ44 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ44

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV5D HA-Δ71 PPM1H

    Plasmid

    #207340

    Purpose

    Mammalian expression of HA-Δ71 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ71

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ACVR1-TSAA

    Plasmid

    #206179

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S192A, S194A

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ACVR1-TAAS

    Plasmid

    #206180

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S192A

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ACVR1-TASA

    Plasmid

    #206181

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S194A

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF0230

    Plasmid

    #143206

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF595 (ZNF595 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28 mePOU-POU

    Plasmid

    #206392

    Purpose

    Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    ePOU
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge