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pFSW Syt1-C2A D230,232N IRES GFP

Plasmid

#133824

Purpose

Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D230,232N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pEGFP.90 beta

Plasmid

#108221

Purpose

Mammalian expression vector for EGFP fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EGFP-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCherry.90 beta

Plasmid

#108223

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-wt

Plasmid

#86078

Purpose

A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-wt

Plasmid

#86075

Purpose

A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_N_CDK8

Plasmid

#111685

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CDK8 (CDK8 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pADSL-EGFP

Plasmid

#99109

Purpose

Expresses human ADSL-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ADSL (ADSL Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Tags

Flag

Expression

Mammalian

Mutation

*

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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GST-Delta23C11orf83

Plasmid

#65849

Purpose

bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of the 23 first aa (TM)

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ20436

Plasmid

#29757

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ20436 (C12orf41 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-BECN1

Plasmid

#99328

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

Beclin 1 (BECN1 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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MAC_C_LAMP1

Plasmid

#111669

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Tags

Halo7-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_EZRI

Plasmid

#111665

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

EZRI (EZR Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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