We narrowed to 20,079 results for: INO

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
SH3PXD2A-PX (1-126)

Plasmid

#119115

Purpose

Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SH3PXD2A-PX (1-126) (SH3PXD2A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_14

Plasmid

#194675

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_14

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_15

Plasmid

#194676

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_15

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

Plasmid

#32737

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RNF110

Plasmid

#126547

Purpose

Expresses GFP-tagged RNF110 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF110 (PCGF2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-E>A-V5

Plasmid

#51298

Purpose

expression of the inactive mutant of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc E247A (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Mutation

E247A, enzymatic inactive mutant

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
sg1+sg2+sg3

Plasmid

#113969

Purpose

Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1+sg2+sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116A

Plasmid

#123081

Purpose

Gateway entry clone encoding human GABARAP G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1026-pMCSG7-HpaCas9

Plasmid

#121540

Purpose

Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

HpaCas9

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-FLAG-NEK9T210A

Plasmid

#168270

Purpose

Expresses kinase-dead NEK9 mutant in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed threonine 210 to alanine

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS1027-pMCSG7-SmuCas9

Plasmid

#121541

Purpose

Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

SmuCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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L-YARS2-9

Plasmid

#166533

Purpose

scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only