We narrowed to 11,576 results for: Vars;

1. pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

   Plasmid

   #101063

   Purpose

   AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2_F391W-G395D
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF-Mm2

   Plasmid

   #197100

   Purpose

   To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   PylRS variant, Pyl tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT4-U6-sgRNA-CMV-EGFP

   Plasmid

   #239587

   Purpose

   Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   Sleeping beauty transposone

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-ERBB2

   Plasmid

   #175846

   Purpose

   Expresses ERBB2

   Depositor

   Evan Braunstein

   Article

   Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

   Plasmid

   #177669

   Purpose

   Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

   Depositor

   Karl Deisseroth

   Article

   Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_RHEB_WT

   Plasmid

   #81257

   Purpose

   Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. sFlt1-i13 V5

   Plasmid

   #86044

   Purpose

   human Soluble Flt1 isoform i13 (terminates in intron 13)

   Depositor

   Christie Thomas

   Article

   Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

   Insert

   human soluble Flt1 isoform i13 (FLT1 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   isoform i13 (terminates in intron 13)

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX303-PCDH7-a

   Plasmid

   #108538

   Purpose

   Expresses human PCDH7 transcript variant a in mammalian cells

   Depositor

   Kathryn O’Donnell

   Article

   Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

   Insert

   PCDH7 transcript variant a (PCDH7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 SARS-CoV-2 S D614G

    Plasmid

    #158075

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST TFR1

    Plasmid

    #169890

    Purpose

    Express TFR1 open reading frame. Lacks IRE regulatory elements

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    TFRC (TFRC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    TFRC cDNA, lacking 3'UTR regulatory elements

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-YBX1

    Plasmid

    #192904

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    YBX1 (YBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106186

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPGR-pTF-dnTCF4

    Plasmid

    #114277

    Purpose

    TET-inducible expression of a dominant negative form of the TCF4 transcription factor

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    deletion of AA 1 to 31

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106180

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV5

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

    Plasmid

    #165082

    Purpose

    gRNA 1 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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