We narrowed to 9,181 results for: mel

1. GAL-p300-501-999

   Plasmid

   #89100

   Purpose

   mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 501-999

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAuroraA-GFP-AURKA-mCherry

   Plasmid

   #99878

   Purpose

   Expression of GFP-AURKA-mCherry in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

   Plasmid

   #170823

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA2
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAuroraA-GFP-AURKA_K162M-mCherry

   Plasmid

   #99880

   Purpose

   Expression of GFP-AURKA K162M-mCherry in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   K162M (kinase-dead)

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdEasy-EGFP-CLASP2 340-1362

   Plasmid

   #24522

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant that retains MT lattice bi…

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLX317-SNAI1

   Plasmid

   #115446

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   SNAI1 (SNAI1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. DRH296: FCK-Optopatch2

   Plasmid

   #51694

   Purpose

   Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   Optopatch2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMito-miRFP703

    Plasmid

    #80000

    Purpose

    Mitochondria labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    cytochrome C oxidase subunit VIII (COX8A Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-CLASP2 512-650

    Plasmid

    #24409

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (512-650) (CLASP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CLASP2 deletion mutant with only MT plus end trac…

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5-HA-p300-DY1399

    Plasmid

    #89095

    Purpose

    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300-D1399Y mutant (EP300 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p300-DY1399

    Promoter

    SV40

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-hLin28B

    Plasmid

    #26358

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog B (LIN28B Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABE8.13-m

    Plasmid

    #136296

    Purpose

    expresses ABE8.13-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIN-Ldha

    Plasmid

    #175939

    Purpose

    knockout mouse Ldha

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Ldha gRNA (Ldha Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL-p300-1000-1600

    Plasmid

    #89101

    Purpose

    mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1000-1600

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8.20-d

    Plasmid

    #136301

    Purpose

    expresses ABE8.20-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GAL-p300-2-300

    Plasmid

    #89098

    Purpose

    mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 2-300

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-7 aSyn C141

    Plasmid

    #108866

    Purpose

    Expresses human WT alpha synuclein with a C-terminal cysteine

    Depositor

    Gabriele Kaminski Schierle

    Article

    Stephens et al bioRxiv 2022.10.04.510646

    Insert

    alpha synuclein (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    C141 mutation to allow C-terminal maleimide dye l…

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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