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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-333

Plasmid

#195467

Purpose

Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP004

Plasmid

#101165

Purpose

E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Nicn1

Plasmid

#92344

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBHM2329

Plasmid

#171686

Purpose

pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

C. neoformans U6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hACE2-cMYC-Flag

Plasmid

#158957

Purpose

Used for expression of human ACE2 with AAV packaging

Depositor

Akiko Iwasaki

Insert

Human ACE2 (ACE2 Human)

Use

AAV

Tags

Flag and MYC

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1

Plasmid

#158775

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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STING-V2

Plasmid

#124264

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus2

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB102

Plasmid

#68556

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Article

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CjABE8e

Plasmid

#189929

Purpose

Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

CjABE8e

Expression

Mammalian

Mutation

CjCas9 D8A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 Ultra-Chili

Plasmid

#106173

Purpose

lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable

Depositor

Ewa Snaar-Jagalska

Insert

dTomato

Use

Lentiviral

Tags

T2A, P2A

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#118296

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pNO183

Plasmid

#101068

Purpose

ppsR2-bphP1 bicistronic operon plasmid

Depositor

Jeffrey Tabor

Article

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Inserts

ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
bphP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

insert guanine at position 430 to fix frameshift …

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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